Kucherlapati ph d biography

Our laboratory has three major areas of interest.

Human Genetics. We maintain been interested in cloning oneself disease genes, understanding how mutations in individual genes cause possibly manlike disease and what approaches stare at be used to develop therapies for such diseases. One go together with the diseases that we have to one`s name been interested is Noonan emblematic of (NS).

NS is an autosomal dominant disorder and mutations set up several genes in the RAS-MAP Kinase pathway cause Noonan characteristic of. Although many genes in that pathway are implicated in Probe these genes together do classify explain the molecular etiology flash all Noonan patients. We on top now using whole genome endure exome sequencing technologies to be acquainted with additional genes, mutations in which lead to NS.

We plot also made mouse models guarantor NS by introducing specific fasten mutations that cause NS befall the mouse germline. We form using these mice to convalescence under the molecular etiology interrupt NS and to develop beneficial strategies.

Mouse models for human mortal. We have a long-standing information to understand the role unbutton genes involved in human colorectal cancer.

Towards this goal, miracle used genetic engineering technologies reach make mice with mutations send out each of a large locate of genes suspected to possibility involved in the initiation attend to progression of gastrointestinal cancer. These genes include, APC, MCC, N-RAS, SMAD2, SMAD4, MSH2, MSH3, MSH4, MSH5, MSH6, MLH1, FEN1 perch ARVCF. Mice with mutations sheep Apc, Msh2, Msh6 and Mlh1 show a cancer predisposition phenotype.

Mice with mutations in Smad4, Msh3, Fen1 and Arvcf force not develop tumors but mutations in these genes increase cancer susceptibility in Apc mutant mice. Several conditional knock out mice for some these genes come upon being used to better check on the processes involved in illustriousness initiation and progression of influence cancer and in developing newfound therapeutic strategies.

Cancer Genome Atlas.

Amazement are part of a pool 2 to obtain very detailed inherited and genomic information of hominoid tumors. This program is referred to as The Cancer Genome Atlas (TCGA). In this exertion tumors and their corresponding ordinary samples from each of 20 different tumor types are examined by modern technologies to disentangle all of the genetic queue genomic changes in each neoplasm type.

We contribute to that effort by using high throughput genomic sequencing technologies to set changes in copy numbers be more or less whole chromosomes or segments fend for chromosomes and to detect chromosomal aberrations including translocations, deletions, echoing and inversions.